Bioprospection of actinobacteria derived from freshwater sediments for their potential to produce antimicrobial compounds

Zothanpuia and Ajit Kumar, Passari and Vincent Vineeth, Leo and Preeti Chandra and Brijesh Kumar, and Chandra S., Nayaka and Abeer, Hashem and Abd_Allah, Elsayed Fathi and Alqarawi, Abdulaziz A and Singh, Bhim Pratap (2018) Bioprospection of actinobacteria derived from freshwater sediments for their potential to produce antimicrobial compounds. Microbial Cell Factories, 68 (1). ISSN 1475-2859

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Official URL: http://dox.10.1186/s12934-018-0912-0 PMCID: PMC593...

Abstract

Actinobacteria from freshwater habitats have been explored less than from other habitats in the search for compounds of pharmaceutical value. This study highlighted the abundance of actinobacteria from freshwater sediments of two rivers and one lake, and the isolates were studied for their ability to produce antimicrobial bioactive compounds. 16S rRNA gene sequencing led to the identification of 84 actinobacterial isolates separated into a common genus (Streptomyces) and eight rare genera (Nocardiopsis, Saccharopolyspora, Rhodococcus, Prauserella, Amycolatopsis, Promicromonospora, Kocuria and Micrococcus). All strains that showed significant inhibition potentials were found against Gram-positive, Gram-negative and yeast pathogens. Further, three biosynthetic genes, polyketide synthases type II (PKS II), nonribosomal peptide synthetases (NRPS) and aminodeoxyisochorismate synthase (phzE), were detected in 38, 71 and 29% of the strains, respectively. Six isolates based on their antimicrobial potentials were selected for the detection and quantification of standard antibiotics using ultra performance liquid chromatography (UPLC-ESI-MS/MS) and volatile organic compounds (VOCs) using gas chromatography mass spectrometry (GC/MS). Four antibiotics (fluconazole, trimethoprim, ketoconazole and rifampicin) and 35 VOCs were quantified and determined from the methanolic crude extract of six selected Streptomyces strains.

Item Type: Article
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: lpa manjunath user
Date Deposited: 16 Oct 2019 10:39
Last Modified: 16 Oct 2019 10:40
URI: http://eprints.uni-mysore.ac.in/id/eprint/9223

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