Treatment with anti-NAP monoclonal antibody reduces disease severity in murine model of novel angiogenic protein-induced or ovalbumin-induced arthritis

Nataraj, N. B. and Krishnamurthy, J. and Bharathi P. Salimath (2013) Treatment with anti-NAP monoclonal antibody reduces disease severity in murine model of novel angiogenic protein-induced or ovalbumin-induced arthritis. Clinical and Experimental Immunology, 171 (2). pp. 155-163. ISSN 0009-9104

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Official URL: http://doi.org/10.1111/cei.12009

Abstract

Rheumatoid arthritis (RA) is a polyarticular inflammatory, angiogenic disease. Synovial angiogenesis contributes to inflammation in RA. In this study we have developed an arthritic model in rats using a novel angiogenic protein (NAP), isolated from human synovial fluid of RA patients. We produced anti-NAP monoclonal antibodies (mAbs) and investigated the therapeutic efficacy of the same in adjuvant-induced or NAP-induced arthritis as a model of human RA. The treatment of arthritic rats with anti-NAP mAbs resulted in effective amelioration of paw oedema, radiological arthritic characteristics, serum levels of vascular endothelial growth factor (VEGF) and NAP, compared to that of untreated arthritic animals. Further, profiling of angiogenic markers such as synovial microvessel density, angiogenesis, CD31, VEGF and fms-like tyrosine kinase (Flt1) by immunohistochemistry both in arthritic and anti-NAP mAb-treated animals revealed the efficacy of mAb as an anti-angiogenic functional antibody. Therefore, NAP may be an attractive target to design anti-angiogenic and anti-arthritic therapies to control the pathogenesis of arthritis.

Item Type: Article
Uncontrolled Keywords: adult, Adult, aged, Aged, angiogenesis, Angiogenesis Inducing Agents, angiogenic protein, Animal, animal experiment, animal model, animal tissue, Animals, Antibodies, antirheumatic agent, Arthritis, article, Calcium-Binding Proteins, cardiovascular parameters, CD135 antigen, CD31 antigen, Cells, controlled study, Cultured, Disease Models, Disease Progression, disease severity, drug efficacy, Experimental, female, Female, histopathology, human, human tissue, Humans, immunohistochemistry, Immunotherapy, male, Male, Membrane Proteins, microvasculature, microvessel density, Middle Aged, Monoclonal, monoclonal antibody, nonhuman, novel angiogenic protein, novel angiogenic protein monoclonal antibody, ovalbumin, Ovalbumin, paw edema, priority journal, protein blood level, rat, Rats, Rheumatoid, rheumatoid arthritis, synovial fluid, Synovial Membrane, unclassified drug, vasculotropin, Wistar
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar
Date Deposited: 16 Oct 2019 11:21
Last Modified: 16 Oct 2019 11:23
URI: http://eprints.uni-mysore.ac.in/id/eprint/9203

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