Chronic intermittent stress-induced alterations in the spermatogenesis and antioxidant status of the testis are irreversible in albino rat

Nirupama, M. and Devaki, M. and Nirupama, R. and Yajurvedi, H. N. (2013) Chronic intermittent stress-induced alterations in the spermatogenesis and antioxidant status of the testis are irreversible in albino rat. Journal of Physiology and Biochemistry, 69 (1). pp. 59-68. ISSN 1877-8755

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Abstract

The study was undertaken to find out whether or not chronic stress-induced alterations in spermatogenesis are accompanied by oxidative damage in the testis and reversibility of these effects. Adult male rats (n = 10) were subjected to restraint for 1 h and later after a gap of 4 h to forced swimming exercise for 15 min daily for 60 days and controls (n = 5) were maintained without disturbance. After treatment period, controls and 5 rats in stress group were killed and remaining rats in stress group were maintained without any treatment for 4 months and then autopsied to find out whether effects are reversible or not. The body and testicular weight, total sperm count, and mean number of type A spermatogonia, mid-pachytene spermatocytes, stage 7 spermatids, and elongated spermatids (cellular association in stage VII of spermatogenesis) showed a significant decrease whereas the abnormal sperm count and germ cell apoptosis were increased in stressed and recovery group rats compared to controls. Activities of testicular SOD, CAT, GPx, and GST were significantly decreased whereas MDA levels were significantly increased in stressed rats compared to controls. The SOD, GST, and CAT activities of recovery groups were significantly lower than controls, whereas MDA levels and GPx activity of these rats did not differ from controls. The results, for the first time, reveal that stress-induced loss of germ cells leading to decrease in sperm count may be due to oxidative damage caused by chronic stress and majority of these changes are not reversible.

Item Type: Article
Uncontrolled Keywords: article, male, body weight, controlled study, nonhuman, rat, Animals, catalase, enzyme activity, glutathione transferase, oxidative stress, Rats, superoxide dismutase, Superoxide Dismutase, Wistar, Male, Oxidative Stress, forced swimming test, testis, Testis, apoptosis, Apoptosis, malonaldehyde, Rattus, exercise, spermatozoon count, chronic stress, Organ Size, Stress, Glutathione Peroxidase, Wistar rat, Catalase, glutathione peroxidase, Physiological, Glutathione Transferase, germ cell, Immobilization, pachytene, Sperm Count, spermatid, Spermatids, spermatocyte, Spermatocytes, spermatogenesis, Spermatogenesis, Spermatogonia, spermatogonium, Swimming, testis weight
Subjects: B Life Science > Zoology
Divisions: Department of > Zoology
Depositing User: Arshiya Kousar
Date Deposited: 17 Oct 2019 10:05
Last Modified: 17 Oct 2019 10:05
URI: http://eprints.uni-mysore.ac.in/id/eprint/9190

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