Separation of Ditalimfos organophosphorus fungicide in adsorption thin layer chromatography and reverse phase high performance thin layer chromatography

Nagaraju, P. M. and Bhargavi, S. M. and Praveen, U. S. and Kemparaju, K. (2013) Separation of Ditalimfos organophosphorus fungicide in adsorption thin layer chromatography and reverse phase high performance thin layer chromatography. Journal of Chemical and Pharmaceutical Research, 5 (2). pp. 231-239. ISSN 0975-7384

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Abstract

Poisoning by ditalimfos (D) is although uncommon; recently, a few cases of deaths owing to the ingestion of ditalimfos were reported in our laboratory. The chromatographic separation is of primary interest when it is contemplated from a forensic matrix. Hence, a study was undertaken to separation of ditalimfos by NP-TLC on silica gel 60F254 and RP- HPTLC on silica gel 60RP-18 WF254 plates with varying mobile phase composition 10:0- 6:4. The relationship between the mobile phase composition (n-hexane+acetone for NP-TLC, and methanol+water for RP-HPTLC) and RF values affirmed that with the increase in the acetone content in case of NP-TLC and decrease of the water content in RP-HPTLC resulted in the increase in RF values for the ditalimfos. The relationship between the mobile phase composition and retention parameter RF, HRF and RM was analysed. Under the chromatographic condition ditalimfos has low RM values this indicate the hydrophilicity of the compound. The UV apex of maximum absorption was 200nm for ditalimfos as measured by multiwavelength scan in the UV range, and higher values of peak area and peak height was obtained at the same wavelength. The present study clearly indicate that separation of ditalimfos in different mobile phase composition in NP-TLC and RP-HPTLC.The minimum ∆RF values indicate good separation was accepted as 0.04, when compared to the separation of any one neighbor compound. We can choose the any combination of solvent system where ∆RF 0.04.

Item Type:	Article
Uncontrolled Keywords:	absorption, accuracy, acetone, Acetone, adsorption thin layer chromatography, article, Chromatographic conditions, Chromatographic separations, concentration (parameters), densitometry, ditalimfos, Ditalimfos, fungicide, hexane, High performance thin layer chromatography, hydrophilicity, intermethod comparison, Liquid chromatography, methanol, Mobile-phase composition, Multi-wavelength scans, NP-TLC, Phase composition, process optimization, reverse phase high performance thin layer chromatography, RP-HPTLC, sensitivity analysis, Separation, separation technique, silica gel, Silica gel, Thin layer chromatography, ultraviolet radiation, unclassified drug, water, water content
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	19 Oct 2019 07:16
Last Modified:	19 Oct 2019 07:16
URI:	http://eprints.uni-mysore.ac.in/id/eprint/9160

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