Krishna, H. and Nagaraja, P. and Shivakumar, A. and Chamaraja, N. A. and Aradhana, N. (2013) Quantification and evaluation of kinetic bio-catalytic pathway of horseradish peroxidase in an electron mediated reaction system and its applications in plant extracts. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 102. pp. 75-81. ISSN 1386-1425
Full text not available from this repository. (Request a copy)Abstract
The intermolecular coupling of 2,5-dimethoxyaniline (DMA) as mediated electron transfer reaction in presence of H2O2 and peroxidase in acetate buffer of pH 4.2 resulting green colored product having maximum absorption at λmax = 740 nm was investigated by spectrophotometer. Under optimum conditions, linearity range for the quantification of H2O2 was 2.0-288.0 μM and for peroxidase were 0.59-9.46 and 0.443-9.46 nM by kinetic and fixed-time method, respectively. The catalytic efficiency and catalytic power were KeffD = 2.354 � 105 M-1 min-1 and KpowD = 4.59 � 10-4 min-1, respectively. From the plot of d(1/D o) vs d(1/Vo) and d(1/Ho) vs d(1/V o), Michaelis-Menten constants for DMA and H2O 2were found that KmD = 1458 μM and KmH2O2 = 301 μM. Applicability of the method was tested for peroxidase activity in some plant extracts and compared with guaiacol/peroxidase system. Regarding superiority of the method, it is suggested that DMA/peroxidase system can be a better hydrogen donor for HRP assay than guaiacol system as evident from kinetic data.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | article, 2, plant extract, medicinal plant, hydrogen peroxide, methodology, kinetics, Kinetics, Hydrogen Peroxide, Plant Extracts, metabolism, Spectrophotometry, spectrophotometry, Models, Biological, biological model, Medicinal, Plants, Plant extracts, Mathematical models, aniline derivative, Aniline Compounds, peroxidase, Peroxidase, Electrons, electron, enzymology, enzyme assay, Enzyme Assays, horseradish peroxidase, Peroxidase activities, 5-Dimethoxyaniline, Acetate buffers, Armoracia, Catalytic efficiencies, Catalytic power, Colored products, Electron transitions, guaiacol peroxidase, Horse-radish peroxidase, horseradish, Horseradish Peroxidase, Hydrogen, Hydrogen donor, Intermolecular coupling, Kinetic data, Linearity range, Mediated electron transfer, Mediated reactions, Michaelis-Menten constant, Optimum conditions |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Arshiya Kousar Library Assistant |
| Date Deposited: | 15 Oct 2019 05:20 |
| Last Modified: | 15 Oct 2019 05:20 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/8978 |
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