Hydroxylation of 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid monosodium salt catalysed by horseradish peroxidase and hydrogen peroxide: Computation of kinetic parameters including its application to crude plant extracts

Krishna, H. and Nagaraja, P. and Shivakumar, A. and Avinash, K. and Lingaiah, V. (2013) Hydroxylation of 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid monosodium salt catalysed by horseradish peroxidase and hydrogen peroxide: Computation of kinetic parameters including its application to crude plant extracts. Journal of the Chinese Chemical Society, 60 (4). pp. 452-459. ISSN 0009-4536

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Official URL: http://doi.org/10.1002/jccs.201200351

Abstract

The new spectrophotometric assay method for the quantification of peroxidase activity uses 4-amino-5- hydroxynaphthalene-2,7-disulfonicacid monosodium salt (AHNDSA) as chromogenic co-substrate. The method is based on hydroxylation of AHNDSA in presence of H2O2/peroxidase forming quinone, having λmax = 460 nm in the acetate buffer (pH = 4.0) at 30 °C. The linearity of H2O2 by kinetic method was 10-332 μM and for peroxidase by kinetic and fixed time methods were 1.18-18.92 and 1.18-9.46 nM, respectively. Catalytic efficiency and catalytic power for peroxidase assay were 7.965 � 104M-1min-1 and 3.76 � 10-4 min-1, respectively. Fromthe plot of d(1/Ao) vs d(1/Vo) and d(1/Ho) vs d(1/V o), the apparent Michaelis-Menten constants for H2O 2 and AHNDSA were Km H2O2 = 68 and Km A = 275 μM, respectively. The method was tested with some plant extracts and also compared with guaiacol/peroxidase system. Except Boerhavia diffusa, all other tested plant samples showed highest peroxidase activity. The proposed method is a rapid and convenient method to determine peroxidase activity by spectrophotometer. This method for the first time reports peroxidase activity in Lantana camara and Oplismenus compositus plants. Kinetic results showed that AHNDSA/peroxidase system can be better hydrogen donor for peroxidase assay than guaiacol system.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Arshiya Kousar
Date Deposited: 15 Oct 2019 05:15
Last Modified: 15 Oct 2019 05:15
URI: http://eprints.uni-mysore.ac.in/id/eprint/8976

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