Sri Raghava and Rajeswari Shome and Umesha, S. (2019) Epidemiological studies of Bovine Brucellosis in rural villages of Mandya District Karnataka, India. Research Journal of Life Sciences, Bioinformatics, Pharmaceutical and Chemical Sciences, 5 (4). pp. 159-168. ISSN 2454-6348
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Abstract
Bovine Brucellosis is one of the devastating zoonotic diseases that affects the multiple livestock species and causes great economic losses. Therefore, appropriate detection and characterization of brucella species in farm animals to control the spread of infection, and obtain epidemiological data for planning disease control strategies are required. The present study was conducted to estimate the bovine brucellosis prevalence and possible risk factors associated with it in different villages of Mandya District, Karnataka. Blood and Milk samples collected from 210 animals of different villages of Mandya District Karnataka, were examined by the Rose bengal test (RBT) and Milk ring test(MRT).The overall seropositivity was 4.3 % for RBT and 3.3 % for MRT. The seropositive samples were further validated by the polymerase chain reaction (PCR) and differentiation by Bruce ladder PCR. The prevalence of bovine brucellosis by Polymerase chain reaction was found to be 4.3 %. Bruce ladder polymerase chain reaction showed that the isolated samples are Brucella abortus. This study indicated an urgent need of policy for prevention and control of brucellosis in dairy animals.
Item Type: | Article |
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Subjects: | B Life Science > Biotechnology |
Divisions: | Department of > Biotechnology |
Depositing User: | C Swapna Library Assistant |
Date Deposited: | 12 Oct 2019 06:58 |
Last Modified: | 12 Oct 2019 06:58 |
URI: | http://eprints.uni-mysore.ac.in/id/eprint/8933 |
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