Development of B1 nested PCR for assessing the prevalence of zoonotic protozoan disease agent toxoplasma gondii among food animals from Karnataka state, Southern India

Rajendran, C. and Keerthana, C. M. and Anilakumar, K. R. and Satbige, A. S. and Shubha Gopal (2018) Development of B1 nested PCR for assessing the prevalence of zoonotic protozoan disease agent toxoplasma gondii among food animals from Karnataka state, Southern India. Journal of Microbiology and Laboratory Science, 1 (1). pp. 1-8.

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Abstract

Toxoplasmosis samples meat or portions edible from gondii Toxoplasma of infection natural of detection direct for aimed was study present The. birds intended stalls fish and mutton available locally the from chicken backyard and fishes, goats and sheep as such animals food different of unorganised from was sampling This. India, State Karnataka of part Southern and North from consumption human of purpose the for genomic total the using analysed study This. simultaneously, animals the slaughtering after processing by sold were muttons where stalls fishes of numbers 41), tissues brain and heart, liver (goat of numbers 34, sheep of numbers 110 from extracted) numbers 218 (DNA overall an showed which, method) PCR (reaction chain polymerase nested B1 by chicken backyard of tissues brain of numbers 33 and and% 09.29 revealed chevon and mutton which of, analysed offal and meat various among infection gondii.T% 47.22 of prevalence among infection gondii.T of prevalence higher a is there that evidence showing study present the of results The. respectively%, 23.38 %100 revealed PCR nested B1 designed the, Further. beings human to threat a directly pose may which, consumption human for meat .DNA gondii.T positive the with specificity% 100 and sensitivity.

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Arshiya Kousar
Date Deposited: 05 Oct 2019 09:37
Last Modified: 05 Oct 2019 09:37
URI: http://eprints.uni-mysore.ac.in/id/eprint/8759

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