Evaluation of cleome gynandra for its chemical composition, antioxidant potential and detection of flavonoids using thin layer chromatography

Deepashree, C. L. and Shubha, G. (2014) Evaluation of cleome gynandra for its chemical composition, antioxidant potential and detection of flavonoids using thin layer chromatography. International Journal of Scientific Research, 3 (10). ISSN 2277 - 8179

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Abstract

Cleome gynandra a well known medicinal plant with known anti-inflammatory activity was studied for its antioxidant property and preliminary detection of compounds responsible for its antioxidant potential. Non polar and polar extracts of Cleome gynandra was subjected to various preliminary phytochemical screening for the detection of phytochemicals. Various in vitro antioxidant method like DPPH radical scavenging assay, Reducing power assay and Metal chelation assay was conducted to evaluate its antioxidant potential and active principle responsible for antioxidant activity was screened using Thin Layer Chromatography. Preliminary phytochemical analysis revealed the presence of flavonoid, alkaloid, terpenoid, steroid and tannins in different solvents. Both methanol and acetone extract proved the best antioxidant activity when compared with other extracts. And Thin Layer Chromatography guided detection showed that active component of the methanol extract was found to be flavonoid and needs further compound identification. Thus it could serve as antioxidant drug in future.

Item Type: Article
Uncontrolled Keywords: Cleome Gynandra and Solvent Extracts and Phytochemicals Screening and Antioxidant Activity
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Shrirekha N
Date Deposited: 04 Oct 2019 10:32
Last Modified: 23 Dec 2020 06:13
URI: http://eprints.uni-mysore.ac.in/id/eprint/8722

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