Interaction of Listeria monocytogenesandstaphylococcus epidermidisin dual species biofilms

Zameer, F. and Kreft, J. and Shubha Gopal (2010) Interaction of Listeria monocytogenesandstaphylococcus epidermidisin dual species biofilms. Journal of food safety, 30 (4). pp. 954-968. ISSN 0149-6085

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The development of mixed biofilms ofListeria monocytogenesandSta-phylococcus epidermidiswas studied on two model surfaces. Growth tests at30C for mono and mixed cultures of the two bacteria in defined Hsiang-NingTsai Medium, in tryptic soy broth (TSB)+1% glucose, TSB+0.6% yeastextract (TSBYE) and TSBYE+1% glucose was performed. The optimalgrowth was seen in TSBYE+1% glucose, and hence, biofilm formation in thismedium was studied using a polystyrene microtiter plate assay. Mono andmixed cultures with different ratios of the two bacteria were assayed for (1)biofilm strength, expressed as the amount of cell-bound crystal violet and (2)amount of matrix polysaccharide measured by ruthenium red staining. Themixture with a 40:60 ratio ofL. monocytogenestoS. epidermidisyielded thehighest values in both assays. The biofilm strength measured for this ratioindicated a synergistic interaction between the two bacterial species. Scan-ning electron microscopy of biofilms formed on cellulose membranes alsorevealed such an interaction.

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 04 Oct 2019 10:23
Last Modified: 04 Oct 2019 10:24

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