Transcriptional analysis of Thiol Disulfide Redox Metabolism (TDRM) genes in Listeria monocytogenes in biofilm and planktonic forms grown at different temperatures

Zameer, F. and Shubha Gopal (2010) Transcriptional analysis of Thiol Disulfide Redox Metabolism (TDRM) genes in Listeria monocytogenes in biofilm and planktonic forms grown at different temperatures. International Journal of Pure and Applied Sciences, 4 (1). pp. 21-27.

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Abstract

Listeria monocytogenes is a food-borne pathogen, which tolerates a wide range of stress conditions. The organism being robust, its stress adaptation strategies are poorly understood. The thiol disulfide redox metabolic pathway plays a pivotal role in combating the stress response induced during growth environments, especially in biofilms wherein the bacterium gains the ability to withstand various environmental stress factors. So far the effect of different temperatures on genes involved in thiol disulfide redox metabolism (TDRM) in relation to biofilm and planktonic cells has not yet been studied in L. monocytogenes. This study aims to understand the involvement of specific TDRM genes encoding for thioredoxin, glutathione, peroxide and their transcriptional regulator systems using Real-Time PCR (Qrt-PCR) for planktonic and biofilm cells grown at different temperature of 18 0 C, 25 0C, 30 0C and 37 0C. The results obtained suggest that TDRM genes analyzed were highly regulated in mature biofilms than their planktonic counterpart at 30 0C. Furthermore, rpoB, a RNA polymerase beta-subunit, was standardized as internal control for optimization of qRT-PCR. The transcriptome analysis could explain the thiol disulfide redox metabolism in combating stress, which contributes to bacterial survival in food processing environments and host-pathogen interactions.

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Arshiya Kousar
Date Deposited: 04 Oct 2019 09:51
Last Modified: 04 Oct 2019 09:51
URI: http://eprints.uni-mysore.ac.in/id/eprint/8710

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