Applications of polymerase chain reaction: Single strand conformation polymorphism to diagnose disease causing microorganisms

Umesha, S. and Chandrashekar, S. and Chandan, S. (2011) Applications of polymerase chain reaction: Single strand conformation polymorphism to diagnose disease causing microorganisms. Biotechnology: An Indian Journal, 5 (2). pp. 100-107. ISSN 0974-7435

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Abstract

Diagnosis of disease causing microorganisms is still primarily based on conventional methods viz., staining, cultures and biochemical analyses etc.,A series of molecular techniques such as Polymerase Chain Reaction- Single Strand Conformation polymorphism(PCR-SSCP), Random Amplified polymorphic DNA (RAPD), Restriction Fragment Length Polymorphism( RFLP),Amplified Fragment Length Polymorphism(AFLP) etc., are increasingly introduced and incorporated in the clinical microbiology laboratories world wide. These techniques are, rapid and offer high sensitivities, screening method which is cost-effective and the entire procedure takes around 6 to 7 h only, and hundreds of samples can be screened at a time.We have attempted to consolidate the literature about the utilization of PCR-SSCP technique in diagnosing the disease causing microorganisms.

Item Type: Article
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: MUL SWAPNA user
Date Deposited: 04 Oct 2019 05:32
Last Modified: 04 Oct 2019 05:32
URI: http://eprints.uni-mysore.ac.in/id/eprint/8679

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