Sensitive spectrophotometric methods for the determination of Gatifloxacin in pharmaceuticals using Bromate-Bromide, Methylene blue and Rhodamine-B as reagents

Basavaiah, K. and Anil Kumar, U. R. (2007) Sensitive spectrophotometric methods for the determination of Gatifloxacin in pharmaceuticals using Bromate-Bromide, Methylene blue and Rhodamine-B as reagents. Journal of Chemistry, 4 (2). pp. 173-179. ISSN 2090-9071

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Official URL: https://dx.doi.org/10.1155/2007/808130

Abstract

A simple spectrophotometric method is proposed for the determination of zidovudine(ZDV) in bulk drug and in pharmaceutical preparations. The method is based on the oxidation of ZDV by a known excess of oxidant N-bromosuccinimide (NBS), in buffer medium of pH 1.5, followed by the estimation of unreacted amount of oxidant with metol and sulphanilic acid. The reacted oxidant corresponds to the amount ZDV. The purple-red reaction product absorbs maximally at 530 nm and Beer’s law is obeyed over a range 5 to 75 μg mL-1. The apparent molar absorptivity is calculated to be 5.1×103 L mol-1cm-1, and the corresponding Sandell sensitivity value is 0.052 μg cm-2. The limit of detection and quantification are found to be 0.90 and 2.72, respectively. Intra-day and inter-day precision and accuracy of the developed methods were evaluated as per the current ICH guidelines. The method was successfully applied to the assay of ZDV in tablet/capsule preparations and the results were statistically compared with those of the reference method by applying the Student’s t-test and F-test. No interference was observed from the common tablet/capsule excipients. The accuracy of the method was further ascertained by performing recovery studies via standard-addition method.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: MUL SWAPNA user
Date Deposited: 26 Sep 2019 10:13
Last Modified: 26 Sep 2019 10:13
URI: http://eprints.uni-mysore.ac.in/id/eprint/8565

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