Kini, K. Ramachandra and Vasanthi, N. S. and Umesh Kumar, S. and Shetty, H. S. (2000) Purification and properties of a major isoform of beta-1,3-glucanase from pearl millet seedlings. Plant Science, 150 (2). pp. 139-145. ISSN 0168-9452
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A major isoform of beta-1,3-glucanase from pearl miller seedlings was purified following ammonium sulfate precipitation, ion-exchange chromatography and gel filtration techniques. The enzyme had a molecular weight of 20.5 kDa on SDS-PAGE and was highly basic with a pI of 9.6. It was thermostable with a broad temperature optima for activity ranging from 37 to 70 degrees C and had an optimum pH of 5.2. Mercuric chloride and para-chloromercuric benzoate inhibited completely the enzyme while manganese chloride activated it. Antibodies raised against the purified beta-1,3-glucanase identified another protein with an apparent molecular weight of 30 kDa in western reactions. Significance of this enzyme in pearl millet-downy mildew host-pathogen interaction is discussed. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | beta-1,3-glucanase; pearl miller; downy mildew; host-pathogen interaction |
| Subjects: | B Life Science > Biotechnology |
| Divisions: | Department of > Biotechnology |
| Depositing User: | Users 19 not found. |
| Date Deposited: | 25 Sep 2019 09:22 |
| Last Modified: | 22 Jul 2022 05:51 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/8520 |
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