Silymarin potentiates the anti-inflammatory effects of Celecoxib on chemically induced osteoarthritis in rats

Ashkavand, Z. and Malekinejad, H. and Amniattalab, A. and Rezaei-Golmisheh, A. and Vishwanath, B. S. (2012) Silymarin potentiates the anti-inflammatory effects of Celecoxib on chemically induced osteoarthritis in rats. PHYTOMEDICINE, 19 (13). pp. 1200-1205.

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Official URL: http://dx.doi.org/10.1016/j.phymed.2012.07.008

Abstract

Silymarin (SMN) is used as an antioxidant complex to attenuate the pro-oxidant effects of toxic agents. This study was carried out to investigate the effect of SMN, Celecoxib (CLX) individually and in combination on monoiodoacetate (MIA)-induced osteoarthritis (OA) in rat. Forty adult Wistar rats were assigned to control and test groups. Animals in the test group following OA induction were subdivided into 4 subgroups according to the treatment profile: OA(+); received saline normal (5 ml/kg, b.w.), OA(+)CLX(+); received CLX (100 mg/kg, orally), OA(+)SMN(+), received SMN (50 mg/kg, orally), and OA(+)CLX(+)SMN(+), received both CLX and SMN. The animals received test compounds by gastric gavage for 14 consecutive days. Animals in the OA(+) group showed a significant (p < 0.01) increase in serum and synovial levels of IL-1 beta, while both test compounds reduced the IL-1 beta level. Both CLX and SMN lowered the OA-increased level of malondialdehyde by 77% and 79% and nitric oxide by 73% and 76%, respectively, in the synovial tissue. Special safranin O (SO) histopathological staining revealed that CLX and SMN improved the MIA-induced destruction and fibrillation in cartilage surface. CLX and SMN regulated the MIA-up regulated IL-1 beta at mRNA level. The combination therapy resulted in an additive effect between CLX and SMN in biochemical, histopathological and molecular assays. These findings suggest that SMN exerts anti-inflammatory effect and also potentiates the anti-inflammatory effect of CLX on MIA-induced OA. The anti-inflammatory property of SMN may attribute to its antioxidant capacity, which affects the proinflammatory mediators at translational and transcriptional level. (c) 2012 Elsevier GmbH. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Silymarin; Antioxidant; Interleukin-1 beta; Osteoarthritis; Proinflammatory mediators
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Biochemistry
Depositing User: Users 7 not found.
Date Deposited: 08 May 2013 07:26
Last Modified: 09 May 2013 09:15
URI: http://eprints.uni-mysore.ac.in/id/eprint/8427

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