Spectrophotometric determination of metronidazole and tinidazole in pharmaceutical preparations

Nagaraja, P. and Sunitha, K. R. and Vasantha, R. A. and Yathirajan, H. S. (2002) Spectrophotometric determination of metronidazole and tinidazole in pharmaceutical preparations. Journal of Pharmaceutical and Biomedical Analysis, 28 (3). 527 - 535. ISSN 1873-264X

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Abstract

Sensitive and simple spectrophotometric methods for the determination of metronidazole (MNZ) and tinidazole (TNZ) in either pure form or in its pharmaceutical formulations are described. The first method is based on the interaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with MNZ/TNZ (reduced drug) in presence of copper sulphate and pyridine in acidic medium. The resulting yellowish orange products have λmax of 500 and 490 nm, respectively, for MNZ and TNZ and are stable for about 4 h. The second method describes the reaction between reduced diazotised drugs with N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) in neutral medium to yield pink products which have λmax of 520 and 505 nm, respectively, for MNZ and TNZ, respectively. The products are stable for more than 24 h. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method. Both the methods are highly reproducible and have been applied to a wide variety of pharmaceutical preparations and the results compare favourably with those of official methods.

Item Type:	Article
Uncontrolled Keywords:	Metronidazole, Tinidazole, MBTH, NEDA, Diazotisation, Spectrophotometry
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Manjula P Library Assistant
Date Deposited:	23 Sep 2019 11:22
Last Modified:	23 Sep 2019 11:22
URI:	http://eprints.uni-mysore.ac.in/id/eprint/8394

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