Rapid clonal propagation of nothapodytes foetida (Wight) sleumer: A threatened medicinal tree

Ravishankar Rai, V. (2002) Rapid clonal propagation of nothapodytes foetida (Wight) sleumer: A threatened medicinal tree. In Vitro Cellular & Developmental Biology - Plant, 38 (4). pp. 347-351. ISSN 1475-2689

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Official URL: https://doi.org/10.1079/IVP2002300

Abstract

Nothapodytes foetida (Wight) is a small evergreen tree and the extract from this tree is used to make the antileukaemia and antitumoral compound camptothecin. Due to exploitation of this resource, efficient methods for rapid propagation of N. foetida are highly desirable. Multiple shoots were induced on hypocotyl segments of 20–25-d-old seedlings of N. foetida cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of cytokinins. The highest shoot multiplication was achieved on MS medium containing thidiazuron (TDZ) at the concentration of 2.2 μM. Inhibition of shoot elongation by TDZ was overcome by transferring shoot cultures to medium containing 2.2 μM benzylaminopurine which produced healthy shoots after three additional subcultures. The production of shoots was further promoted by repeated subculturing of original explants on fresh multiplication medium after each harvesting of the newly formed shoots. In vitro rooting was best induced (87%) in shoots excised from proliferated shoot cultures on one-fourth MS medium augmented with 5.7 μM indole-3-acetic acid and 2.4 μM indolebutryic acid (IBA). In vitro-developed shoots were also rooted ex vitro by dipping in 49 μM IBA for 10 min. In vitro- and ex vitro-rooted plants were successfully acclimatized and established in greenhouse conditions.

Item Type: Article
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: manjula User
Date Deposited: 23 Sep 2019 07:26
Last Modified: 23 Sep 2019 07:26
URI: http://eprints.uni-mysore.ac.in/id/eprint/8366

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