Nagaraja, P. and Vasantha, R. A. and Yathirajan, H. S. (2002) Spectrophotometric determination of folic acid in pharmaceutical preparations by coupling reactions with iminodibenzyl or 3-aminophenol or sodium molybdate–pyrocatechol. Analytical Biochemistry, 307 (2). 316 - 321. ISSN 1096-0309
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Novel coupling reagents are used for the simple and sensitive spectrophotometric determination of folic acid either in pure form or in its pharmaceutical preparations. The methods are based on the probable diazotization of the p-aminobenzoylglutamic acid obtained after reductive clevage of folic acid, followed by either coupling with iminodibenzyl to give a violet product with λmax of 580nm or coupling with 3-aminophenol to produce an orange yellow-colored product with λmax of 460nm. Sodium molybdate and pyrocatechol are used in the third method and the pale red-colored product formed has a λmax of 490nm. The methods are highly reproducible and have been applied to the determination of folic acid in tablets and the results compare favorably with the official method. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed methods.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Folic acid, Spectrophotometry, Iminodibenzyl, 3-Aminophenol, Pyrocatechol, Sodium molybdate, Pharmaceuticals |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Manjula P Library Assistant |
| Date Deposited: | 23 Sep 2019 06:20 |
| Last Modified: | 23 Sep 2019 06:20 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/8356 |
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