Purification and partial characterization of manganese superoxide dismutase from downy mildew resistant pearl millet seedlings inoculated with Sclerospora graminicola

Babitha, M. P. and Prakash, H. S. and Shekar Shetty, H. (2002) Purification and partial characterization of manganese superoxide dismutase from downy mildew resistant pearl millet seedlings inoculated with Sclerospora graminicola. Plant Science, 163 (4). 917 - 924. ISSN 1873-2259

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Official URL: https://doi.org/10.1016/S0168-9452(02)00238-8

Abstract

The Mn-SOD was purified from pearl millet by ammonium sulfate precipitation followed by column chromatography using DEAE-cellulose and Sephadex G-100. The isozyme has a molecular weight of 35 kDa. Electrophoresis revealed a single band of SOD activity corresponding to the purified enzyme. The purified enzyme was stable over a pH range of 7.0–9.0 for 24 h, and a temperature range of 20–35°C. The purified pearl millet SOD exhibited insensitivity to hydrogen peroxide and cyanide, which is typical of Mn-containing SOD. SOD was purified 73-fold from pearl millet.

Item Type: Article
Uncontrolled Keywords: Superoxide dismutase, Pearl millet, Downy mildew, Host–pathogen interaction
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: manjula User
Date Deposited: 20 Sep 2019 09:49
Last Modified: 20 Sep 2019 09:49
URI: http://eprints.uni-mysore.ac.in/id/eprint/8315

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