Molecular mechanisms of anti-angiogenic effect of curcumin

Anupama E. Gururaj and Madesh, B. and Deepak, A. V. and Marmé, Dieter and Bharathi P. Salimath (2002) Molecular mechanisms of anti-angiogenic effect of curcumin. Biochemical and Biophysical Research Communications, 297 (4). 934 - 942. ISSN 1090-2104

Full text not available from this repository. (Request a copy)
Official URL: https://doi.org/10.1016/S0006-291X(02)02306-9

Abstract

Modulation of pathological angiogenesis by curcumin (diferuloylmethane), the active principle of turmeric, seems to be an important possibility meriting mechanistic investigations. In this report, we have studied the effect of curcumin on the growth of Ehrlich ascites tumor cells and endothelial cells in vitro. Further, regulation of tumor angiogenesis by modulation of angiogenic ligands and their receptor gene expression in tumor and endothelial cells, respectively, by curcumin was investigated. Curcumin, when injected intraperitoneally (i.p) into mice, effectively decreased the formation of ascites fluid by 66 in EAT bearing mice in vivo. Reduction in the number of EAT cells and human umbelical vein endothelial cells (HUVECs) in vitro by curcumin, without being cytotoxic to these cells, is attributed to induction of apoptosis by curcumin, as is evident by an increase in cells with fractional DNA content seen in our results on FACS analysis. However, curcumin had no effect on the growth of NIH3T3 cells. Curcumin proved to be a potent angioinhibitory compound, as demonstrated by inhibition of angiogenesis in two in vivo angiogenesis assay systems, viz. peritoneal angiogenesis and chorioallantoic membrane assay. The angioinhibitory effect of curcumin in vivo was corroborated by the results on down-regulation of the expression of proangiogenic genes, in EAT, NIH3T3, and endothelial cells by curcumin. Our results on Northern blot analysis clearly indicated a time-dependent (0–24h) inhibition by curcumin of VEGF, angiopoietin 1 and 2 gene expression in EAT cells, VEGF and angiopoietin 1 gene expression in NIH3T3 cells, and KDR gene expression in HUVECs. Further, decreased VEGF levels in conditioned media from cells treated with various doses of curcumin (1μM–1mM) for various time periods (0–24h) confirm its angioinhibitory action at the level of gene expression. Because of its non-toxic nature, curcumin could be further developed to treat chronic diseases that are associated with extensive neovascularization.

Item Type: Article
Uncontrolled Keywords: Curcumin, Anti-angiogenesis, Angiogenic ligands/receptors/gene expression, Apoptosis
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: manjula User
Date Deposited: 20 Sep 2019 09:46
Last Modified: 03 Oct 2019 10:30
URI: http://eprints.uni-mysore.ac.in/id/eprint/8313

Actions (login required)

View Item View Item