Sensitive bromatometric methods for the determination of salbutamol sulphate in pharmaceuticals

Somashekar, B. C. and Basavaiah, K. (2007) Sensitive bromatometric methods for the determination of salbutamol sulphate in pharmaceuticals. Journal of Analytical Chemistry, 62 (5). pp. 432-437. ISSN 0044-4502

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The proposed methods allow semimicro and microlevel determination of SBS in authentic samples and in dosage forms. The spectrophotometric method is much more sensitive than most of the procedures known for the determination of SBS. The titrimetric method takes less than 15 min for analysis. All the procedures are simple and do not need elaborate treatment or tedious extractions. One titrimetric and two spectrophotometric methods are described for the assay of sulbutamol sulphate (SBS) in bulk drugs and in tablets using bromate-bromide mixture and two dyes, rhodamine B and methylene blue, as reagents. In titrimetry, an aqueous solution of SBS is treated with a measured excess of bromate-bromide mixture in a HCl medium, followed by iodometric determination of unreacted bromine. Spectrophotometric methods involve the addition of a known excess of bromate-bromide mixture to SBS in an acid medium, followed by the determination of residual bromine by reacting with either a fixed amount of rhodamine-B and measuring the absorbance at 555 nm (method A) or methylene blue and measuring the absorbance at 665 nm (method B). In all methods, the amount of in situ generated bromine reacted corresponds to the amount of SBS. The titrimetric method is applicable over the 3.0--8.0 mg range and the reaction stoichiometry is found to be 1: 2 (SBS: KBrO3). In spectrophotometric methods, the absorbance is found to increase linearly with the concentration of SBS which is corroborated by the correlation coefficient of 0.9978 and 0.9991 for method A and method B, respectively. The systems obey Beer's law for 0.25--2.5 μg/mL (method A) and 0.75--7.5 μg/mL (method B). The calculated apparent molar absorptivity values are found to be 8.96 × 104 and 4.67 × 104 L mol−1 cm−1, for method A and method B, respectively, and the corresponding Sandell sensitivity values are 6.43 and 12.34 ng/cm2. The limits of detection and quantification are also reported for both spectrophotometric methods. Intraday and interday precision and accuracy of the methods were evaluated. The methods were successfully applied to the assay of SBS in tablet and capsule preparations and the results were compared with those of a reference method by applying Student's t-and F-tests. No interference was observed from common pharmaceutical ajuvants.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: C Swapna Library Assistant
Date Deposited: 17 Sep 2019 06:04
Last Modified: 17 Sep 2019 06:04

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