Detection of phomopsis azadirachtae from dieback affected neem twigs, seeds, embryo by polymerase chain reaction

Nagendra Prasad, M. N. and Bhat, Shankara S. and Raj, Charith A. P. and Janardhana, G. R. (2009) Detection of phomopsis azadirachtae from dieback affected neem twigs, seeds, embryo by polymerase chain reaction. Archives of Phytopathology and Plant Protection, 42 (2). pp. 124-128.

Full text not available from this repository. (Request a copy)
Official URL: https://doi.org/10.1080/03235400600982584

Abstract

Twigs and seeds of Azadirachta indica (Neem) infected with dieback disease, collected from different regions of Karnataka, India were analysed to determine the pathogens. Phomopsis azadirachtae, the causal agent of dieback disease of neem was found to be seed borne. Phomopsis azadirachtae was isolated on PDA and MEA from dieback-infected neem twigs, seeds and embryo. Phomopsis genus-specific primers (5.8S r-DNA) were then used for the detection of Phomopsis azadirachtae, the causative agent of dieback of neem by polymerase chain reaction (PCR). Reactions were performed on DNA isolated from twigs, seeds and embryo of dieback-affected neem trees. Studies revealed the amplification of expected 141 bp DNA in Phomopsis azadirachtae isolated from various parts of diseased trees indicating the causal organism of dieback disease on neem. Isolation and identification by conventional technique takes around 15 – 21 days, whereas the present technique is capable of detecting very low propogules within 4 – 5 days.

Item Type: Article
Uncontrolled Keywords: Neem and Phomopsis azadirachtae and 5 8S r-DNA and dieback and polymerase chain reaction
Subjects: B Life Science > Botany
Divisions: Department of > Botany
Depositing User: Shrirekha N
Date Deposited: 12 Sep 2019 10:41
Last Modified: 12 Sep 2019 10:41
URI: http://eprints.uni-mysore.ac.in/id/eprint/8087

Actions (login required)

View Item View Item