Highly sensitive reaction of tryptophan with p-phenylenediamine

Nagaraja, P. and Yathirajan, H. S. and Vasantha, R. A. (2003) Highly sensitive reaction of tryptophan with p-phenylenediamine. Analytical Biochemistry, 312 (2). 157 - 161. ISSN 1096-0309

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Official URL: https://doi.org/10.1016/S0003-2697(02)00504-3


A simple and sensitive spectrophotometric method for the determination of tryptophan (TRP) is described. The method is based on the coupling reaction of tryptophan with diazotized p-phenylenediamine dihydrochloride (PPDD) in sulfuric acid medium to give the colored product having an absorption maximum at 520nm. The coupled product was stable for 2h. Beer’s law is obeyed in the tryptophan concentration range of 0.25–11μg/ml. The method is applied for the analysis of pharmaceutical preparations of tryptophan and also in protein samples for tryptophan. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method and the significant feature of the method is that most of the amino acids do not interfere in the determination of tryptophan.

Item Type: Article
Uncontrolled Keywords: Tryptophan, -Phenylenediamine, Diazotization, Spectrophotometry, Pharmaceuticals, Protein hydrolysates
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: LA manjunath user
Date Deposited: 12 Sep 2019 07:33
Last Modified: 15 Oct 2019 05:44
URI: http://eprints.uni-mysore.ac.in/id/eprint/8080

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