Crocin, a dietary colorant mitigates cyclophosphamide-induced organ toxicity by modulating antioxidant status and inflammatory cytokines

Jnaneshwari, S. and Hemshekhar, M. and Santhosh, M. S. and Sunitha, K. and Thushara, R. and Thirunavukkarasu, C. and Kemparaju, K. and Girish, K. S. (2013) Crocin, a dietary colorant mitigates cyclophosphamide-induced organ toxicity by modulating antioxidant status and inflammatory cytokines. Journal of Pharmacy and Pharmacology, 65 (4). pp. 604-614.

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Official URL: http://doi.org/10.1111/jphp.12016

Abstract

Objectives This study investigated the protective efficacy of crocin against hepatotoxicity induced by cyclophosphamide (CP) in Wistar rats. Methods The experimental rats were treated with crocin orally at a dose of 10 mg/kg for 6 consecutive days after the administration of a single intraperitoneal dose of CP (150 mg/kg). The ameliorative effect of crocin on organ toxicity was studied by evaluating oxidative stress enzymes, inflammatory cytokines and histological sections. Key Findings A single intraperitoneal CP injection significantly elevated endogenous reactive oxygen species and oxidation of lipids and proteins, which are the hallmarks of oxidative damage in liver and serum. In consequence, the primary defensive reduced glutathione, total thiol and antioxidant enzymes such as superoxide dismutase, catalase, glutathione-S- transferase and glutathione peroxidase, were significantly reduced. In addition, liver and serum aspartate aminotransferase and alanine aminotransferase along with acid and alkaline phosphatase were considerably increased. Oral administration of crocin significantly rejuvenated all the above altered markers to almost normal state. The protective efficacy of crocin was further supported by the histological assessment and restoration of CP-induced inflammatory cytokines and enzyme levels compared with the control drug. Conclusion The results obtained suggest the protective nature of crocin against CP-induced oxidative damage/inflammation and organ toxicity. © 2013 The Authors. JPP © 2013. Royal Pharmaceutical Society.

Item Type: Article
Uncontrolled Keywords: article, histopathology, animal experiment, controlled study, drug efficacy, nonhuman, rat, antioxidant activity, protein, alanine aminotransferase, alanine aminotransferase blood level, Animals, Antioxidants, aspartate aminotransferase, aspartate aminotransferase blood level, Carotenoids, catalase, crocin, Cytokines, glutathione, glutathione transferase, Liver, liver protection, oxidative stress, Rats, reactive oxygen metabolite, superoxide dismutase, thiol, Wistar, alkaline phosphatase, alkaline phosphatase blood level, animal tissue, Drug-Induced Liver Injury, Hydrogen Peroxide, Oxidative Stress, Anti-Inflammatory Agents, Antineoplastic Agents, lipid oxidation, Lipid Peroxidation, Protein Carbonylation, Reactive Oxygen Species, spleen, silymarin, thiobarbituric acid reactive substance, cyclophosphamide, lipid, single drug dose, Biological Markers, liver, cytokine, Oxidoreductases, drug toxicity, Alkylating, Cyclophosphamide, kidney, glutathione peroxidase, Non-Steroidal, cyclophosphamide induced organ toxicity, Food Coloring Agents, Post-Translational, Protein Processing
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Arshiya Kousar
Date Deposited: 13 Sep 2019 05:43
Last Modified: 13 Sep 2019 05:43
URI: http://eprints.uni-mysore.ac.in/id/eprint/8029

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