Characterization of rhizosphere fungi that mediate resistance in tomato against bacterial wilt disease

Jogaiah, S. and Abdelrahman, M. and Tran, L. S. P. and Shin-Ichi, I. (2013) Characterization of rhizosphere fungi that mediate resistance in tomato against bacterial wilt disease. Journal of Experimental Botany, 64 (12). pp. 3829-3842. ISSN 0022-0957

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Official URL: http://doi.org/10.1093/jxb/ert212

Abstract

Plant immunization for resistance against a wide variety of phytopathogens is an effective strategy for plant disease management. Seventy-nine plant growth-promoting fungi (PGPFs) were isolated from rhizosphere soil of India. Among them, nine revealed saprophytic ability, root colonization, phosphate solubilization, IAA production, and plant growth promotion. Seed priming with four PGPFs exhibited early seedling emergence and enhanced vigour of a tomato cultivar susceptible to the bacterial wilt pathogen compared to untreated controls. Under greenhouse conditions, TriH-JSB27 and PenC-JSB41 treatments remarkably enhanced the vegetative and reproductive growth parameters. Maximum NPK uptake was noticed in TriH-JSB27-treated plants. A significant disease reduction of 57.3% against Ralstonia solanacearum was observed in tomato plants pretreated with TriH-JSB27. Furthermore, induction of defence-related enzymes and genes was observed in plants pretreated with PGPFs or inoculated with pathogen. The maximum phenylalanine ammonia lyase (PAL) activity (111U) was observed at 24h in seedlings treated with TriH-JSB27 and this activity was slightly reduced (99U) after pathogen inoculation. Activities of peroxidase (POX, 54U) and β-1,3-glucanase (GLU, 15U) were significantly higher in control plants inoculated with pathogen after 24h and remained constant at all time points. A similar trend in gene induction for PAL was evident in PGPFs-treated tomato seedlings with or without pathogen inoculation, whereas POX and GLU were upregulated in control plus pathogen-inoculated tomato seedlings. These results determine that the susceptible tomato cultivar is triggered after perception of potent PGPFs to synthesize PAL, POX, and GLU, which activate defence resistance against bacterial wilt disease, thereby contributing to plant health improvement.

Item Type: Article
Uncontrolled Keywords: article, India, Fungi, fungus, metabolism, gene expression regulation, Lycopersicon esculentum, Plant Proteins, tomato, development and aging, genetics, growth, physiology, vegetable protein, microbiology, classification, Ralstonia solanacearum, plant disease, Plant Diseases, Gene Expression Regulation, peroxidase, Peroxidase, enzymology, Plant, rhizosphere, Rhizosphere, phenylalanine ammonia lyase, Soil Microbiology, 3 beta glucosidase, 3-beta-Glucosidase, bacterial wilt, Bacterial wilt, defence-related enzymes and genes, glucan 1, Glucan 1, growth promotion, induced systemic resistance, Phenylalanine Ammonia-Lyase, phosphate solubilization, plant growth-promoting fungi, tomato.
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar
Date Deposited: 19 Sep 2019 09:58
Last Modified: 19 Sep 2019 09:58
URI: http://eprints.uni-mysore.ac.in/id/eprint/7977

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