Attenuation of adjuvant-induced arthritis by dietary sesamol via modulation of inflammatory mediators, extracellular matrix degrading enzymes and antioxidant status

Hemshekhar, M. and Mohan Thushara, R. and Jnaneshwari, S. and Devaraja, S. and Kemparaju, K. and Girish, K. S. (2013) Attenuation of adjuvant-induced arthritis by dietary sesamol via modulation of inflammatory mediators, extracellular matrix degrading enzymes and antioxidant status. European Journal of Nutrition, 52 (7). pp. 1787-1799. ISSN 1436-6215

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Abstract

Purpose The dietary sesamol is one of the important constituent of sesame seed that has been mainly claimed to combat cardiovascular disease and diabetes, which are the major secondary complications of arthritis. Thus, the present study was designed to evaluate the anti-Arthritic, anti-inflammatory and anti-stress potentials of sesamol. Methods Arthritis was induced using Freund's complete adjuvant to hind paw of experimental rats. The physical and biochemical alterations and its recovery by sesamol were assessed by measuring enzymatic and non-enzymatic mediators. Arthritis-induced inflammation, oxidative stress and their protective by sesamol were measured by determining the levels of pro-inflammatory cytokines and oxidative stress markers. Results In the present study, sesamol was demonstrated to alleviate arthritis-induced cartilage degeneration by mitigating augmented serum levels of hyaluronidase and matrix metalloproteinases (MMP-13, MMP-3 and MMP- 9). It also protected bone resorption by reducing the elevated levels of bone joint exoglycosidases, cathepsin D and tartarate-resistant acid phosphatases. Sesamol also abrogated the non-enzymatic inflammatory markers (TNF, IL- 1b, IL-6, COX-2, PGE2, ROS, and H2O2,) effectively. In addition, sesamol neutralizes arthritis-induced oxidative stress by restoring the levels of reactive oxygen species, lipid and hydro peroxides and sustained antioxidant homeostasis by re-establishing altered activities of superoxide dismutase, catalase and glutathione-s-Transferase. Conclusion Taken together, the study demonstrated the anti-Arthritic, anti-inflammatory, anti-oxidative stress and chondro-protective potentials of sesamol in vivo. Thus, sesamol could be a single bullet that can fight arthritis as well as the secondary complications of arthritis such as cardio vascular disorders and diabetes.

Item Type:	Article
Uncontrolled Keywords:	article, histopathology, animal experiment, animal model, controlled study, drug screening, nonhuman, rat, antiinflammatory activity, antioxidant activity, unclassified drug, acid phosphatase tartrate resistant isoenzyme, alanine aminotransferase, alanine aminotransferase blood level, Animals, antioxidant, Antioxidants, Arthritis, aspartate aminotransferase, aspartate aminotransferase blood level, cartilage degeneration, catalase, cathepsin D, collagenase 3, cyclooxygenase 2, enzyme activity, Experimental, gelatinase B, glutathione transferase, glycosidase, hyaluronidase, hydrogen peroxide, ibuprofen, Inflammation Mediators, interleukin 1beta, interleukin 6, Matrix Metalloproteinase 13, Matrix Metalloproteinase 9, osteolysis, oxidative stress, prostaglandin E2, Rats, reactive oxygen metabolite, stromelysin, superoxide dismutase, Superoxide Dismutase, Wistar, drug mechanism, alkaline phosphatase, alkaline phosphatase blood level, animal tissue, Hydrogen Peroxide, Oxidative Stress, in vivo study, Phenols, Anti-Inflammatory Agents, antiinflammatory agent, dose response, Reactive Oxygen Species, Interleukin-1beta, Rattus, herbaceous agent, Alanine Transaminase, Aspartate Aminotransferases, tumor necrosis factor, morning dosage, Tumor Necrosis Factor-alpha, glycosaminoglycan, extracellular matrix, Extracellular Matrix, Catalase, Benzodioxoles, sesamol, Interleukin-6, antirheumatic agent, Sesamum indicum, Glutathione Transferase, adjuvant arthritis, beta n acetylhexosaminidase A, bone atrophy, chondroprotection, Cyclooxygenase 2, exoglycosidase, inflammatory infiltrate
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	20 Sep 2019 07:22
Last Modified:	17 Dec 2019 11:18
URI:	http://eprints.uni-mysore.ac.in/id/eprint/7962

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