Withaferin a suppresses the expression of vascular endothelial growth factor in ehrlich ascites tumor cells via sp1 transcription factor

Prasanna Kumar, S. and Shilpa, P. and Bharathi, P. (2009) Withaferin a suppresses the expression of vascular endothelial growth factor in ehrlich ascites tumor cells via sp1 transcription factor. Current Trends in Biotechnology and Pharmacy, 3 (2). pp. 138-148. ISSN 2230-7303

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Abstract

In the ayurvedic system of medicine, the medicinal plant, Withania somnifera Dunal (Solanaceae) finds application for numerous ailments including cancer. This herbal plant yields a host of steroidal lactones called withanolides, some of which have shown growth inhibition of human tumor cell lines. Withaferin A amongst these withanolides reportedly is very active in impairing antitumor activity. However; the underlying molecular mechanisms of this activity remains still unclear. In the present study, we have shown that withaferin A inhibited vascular endothelial cell growth factor (VEGF) -induced tube formation by human umbilical vein endothelial cells (HUVECs) and angiogenesis in chick chorioallantoic membrane (CAM) assay. In Ehrlich ascites tumor (EAT) model, the animals treated with withaferin A suppressed in vivo, the peritoneal angiogenesis and microvessel density. When compared to the untreated animals, the withaferin A treated tumor bearing mice showed a decrease in the volume of ascites and tumor cell number. Quantitation of VEGF levels in ascites from withaferin A untreated or treated tumor bearing mice indicated decreased secretion of VEGF in ascites from treated mice, as measured by ELISA. Studies at molecular level revealed that withaferin A inhibits binding of Sp1 transcription factor to VEGF-gene promoter, in Withaferin A suppresses the expression of vascular endothelial growth factor in Ehrlich ascites tumor cells via Sp1 transcription factor Prasanna Kumar S., Shilpa P. and Bharathi P. Salimath* Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore-570006, India. For Correspondent : Salimathuom@rediffmail.com order to exert its antiangiogenic activity. These results clearly indicate the antiangiogenic potential of withaferin A in modulating antitumor activity..

Item Type: Article
Uncontrolled Keywords: Ehrlich ascites tumor and WithaferinA and Angiogenesis and Sp1 and VEGF.i
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Shrirekha N
Date Deposited: 09 Sep 2019 10:13
Last Modified: 09 Sep 2019 10:13
URI: http://eprints.uni-mysore.ac.in/id/eprint/7868

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