Purification of human serum paraoxonase: a simple and rapid method

Mahadesh Prasad, A. J. and Kemparaju, K. and Frank, Elizabeth. A. and D’Souza, Cletus J. M. (2009) Purification of human serum paraoxonase: a simple and rapid method. African Journal of Biochemistry Research, 3 (4). pp. 125-129. ISSN 1996-0778

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Abstract

Paraoxonase/arylesterases (EC.3.1.8.2) is an enzyme found tightly associated with high density lipoprotein particle in serum. Because of its unique enzyme activity, antioxidant property and its role as an anti atherosclerotic molecule, various methods are used for its purification from human serum. Methods involved in its purification are elaborate and complicated. Also the yield and final activity are highly variable. Here, we report a 2 step method of purification involving affinity chromatography on cibacron blue sepharose followed by gel filtration on sephadex G50.The final preparation was 27.7 fold purified compared with the serum and gave a single band in SDS-PAGE by silver staining.

Item Type: Article
Uncontrolled Keywords: Cibacron Blue Sepharose and Paraoxonase and Phenyl Acetate and SDS-PAGE and Silver Staining
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Shrirekha N
Date Deposited: 09 Sep 2019 09:38
Last Modified: 09 Sep 2019 09:38
URI: http://eprints.uni-mysore.ac.in/id/eprint/7852

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