Inhibition of paraoxonase1 by ketone bodies: An invitro study

Austin Richard, S. and D'Souza, C. J. M. (2013) Inhibition of paraoxonase1 by ketone bodies: An invitro study. Asian Journal of Pharmaceutical and Clinical Research, 6 (3). pp. 119-121.

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Objective: In uncontrolled diabetes mellitus, concentration of ketone bodies is elevated to up to 25mM. This study aims to investigate the inhibitory action of ketone bodies on paraoxonase and arylesterase activity of Paraoxonase1. Method: The inhibition of Paraoxonase1 by ketone bodies was determined spectrophotometrically by using paraoxon and phenyl acetate as substrates. Result: Acetone decreased 32% of paraoxonase activity and 23% of arylesterase activity. Sodium-3-hydroxybutyrate showed 20% reduction in paraoxonase activity but it did not affect arylesterase activity. In contrast, neither of the enzyme activities was affected by sodium-acetoacetate. Conclusion: Higher concentration of acetone and sodium-3- hydroxybutyrate contribute to the reduction of Paraoxonase1 activity.

Item Type: Article
Uncontrolled Keywords: acetone, adult, article, aryldialkylphosphatase 1, arylesterase, concentration (parameters), enzymatic assay, enzyme activity, enzyme inhibition, female, glucose, human, hydroxybutyric acid, in vitro study, ketone body, male, molecular dynamics, normal human, paraoxon, phenylacetic acid, sodium 3 hydroxybutyrate, spectrophotometry, unclassified drug
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 11 Sep 2019 05:06
Last Modified: 03 Jun 2023 11:33

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