Rapid ultra-performance liquid chromatography assay of losartan potassium in bulk and formulations

Ahmad, T. J. and Amruth Raj and Radhika, R. T. and Ananda, S. and Made Gowda, N. and Venkatesha, B. M. (2014) Rapid ultra-performance liquid chromatography assay of losartan potassium in bulk and formulations. Journal of Analytical Science and Technology, 5 (33). pp. 1-6. ISSN 2093-3371

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Official URL: https://doi.org/10.1186/s40543-014-0033-2


Background: Losartan potassium is a non-peptide AT1 receptor drug used in the treatment of hypertension. Methods: A simple, rapid, sensitive, and validated isocratic reverse-phase ultra-performance liquid chromatographic (RP-UPLC) method was developed and validated for the determination of losartan potassium (LOS) in bulk drug and tablets. The assay was developed using Waters Acquity BEH C18 (100 mm × 2.1 mm), 1.7-μm column with a mobile phase consisting of a mixture of phosphate buffer (pH 3.2) and acetonitrile (50:50 v/v). Results: An assay with a total run time of only 5 min was developed. The method monitored at 245 nm exhibited linearity over a concentration range of 2.0 to 15.0 μg mL−1 LOS. The limits of detection and quantification (signal-to-noise ratio (S/N) = 10) were found be 0.018 and 0.054 μg mL−1 , respectively. The intraday and interday RSDs were less than 1.0%. The method was validated by the determination of LOS levels in tablets where the percentage on the label claim was 100 ± 2. The accuracy of the method was further ascertained by recovery studies via the standard addition procedure, which yielded satisfactory results. Conclusion: A rapid UPLC assay of LOS in bulk drug and tablets was developed and validated.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Yuvaraj college > Chemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 07 Sep 2019 06:12
Last Modified: 16 Jul 2022 06:34
URI: http://eprints.uni-mysore.ac.in/id/eprint/7727

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