Micropropagation of pseudoxytenanthera stocksii munro

Sanjaya and Rathore, T. S. and Ravishankar Rai, V. (2005) Micropropagation of pseudoxytenanthera stocksii munro. In Vitro Cellular & Developmental Biology - Plant, 41 (3). pp. 333-337. ISSN 1475-2689

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Abstract

An efficient and reproducible procedure for the large-scale propagation of Pseudoxytenanthera stocksii is described. High-frequency multiple shoot induction was achieved from nodal shoot segments collected from superior/elite genotypes on Murashige and Skoog (MS) liquid medium supplemented with 1-naphthaleneacetic acid (NAA; 2.68 μM) and 6-benzylaminopurine (BA; 4.40 μM) at 28±1°C and 60 μmol m−2 s−1 light intensity under 12h photoperiod. In vitro-differentiated shoots were multiplied on MS liquid medium fortified with NAA (2.68 μM), BA (2.21 μM) and additives: ascorbic acid (283.93 μM), citric acid (118.10 μM), cysteine (104.04 μM), and glutamine (342.24 μM). Subculturing was carried out every 2wk on fresh shoot multiplication medium. About 125–150 shoots per culture flask were harvested within 45–50d. In vitro-differentiated shoot clumps (three or four shoots) were successfully rooted on half-strength MS basal liquid medium with indole-3-butyric acid (4.90 μM), BA (0.44 μM), and additives. This is the first report where in vitro- and in vivo-(through tillers) raised clonal plants were acclimatized and established in the field, where they exhibited normal growth.

Item Type:	Article
Subjects:	B Life Science > Biotechnology
Divisions:	Department of > Biotechnology
Depositing User:	Manjula P Library Assistant
Date Deposited:	06 Sep 2019 09:52
Last Modified:	06 Sep 2019 09:52
URI:	http://eprints.uni-mysore.ac.in/id/eprint/7685

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