A Low molecular weight isoform of hyaluronidase: Purification from indian cobra (naja naja) venom and partial characterization

Girish, K. S. and Kemparaju, K. (2005) A Low molecular weight isoform of hyaluronidase: Purification from indian cobra (naja naja) venom and partial characterization. Biochemistry (Moscow), 70 (6). pp. 708-712. ISSN 1608-3040

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Official URL: https://doi.org/10.1007/s10541-005-0172-6

Abstract

A low molecular weight isoform of hyaluronidase (NNH2) has been isolated from Indian cobra (Naja naja) venom by successive chromatography on Sephadex G-75 and CM-Sephadex C-25 columns. The apparent molecular weight determined by SDS-PAGE is 52 kD, and the pI value is 9.7. NNH2 is an endoglycosidase and exhibits in vitro absolute specificity for hyaluronan; it also hydrolyzed hyaluronan in human skin sections. NNH2 is nontoxic, but it indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I. Curcumin, indomethacin, and tannic acid inhibited dose dependently the degradation of hyaluronan by NNH2.

Item Type: Article
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: manjula User
Date Deposited: 05 Sep 2019 10:10
Last Modified: 05 Sep 2019 10:10
URI: http://eprints.uni-mysore.ac.in/id/eprint/7651

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