Procoagulant activity of Calotropis gigantea latex associated with fibrin(ogen)olytic activity

Rajesh, R. and Raghavendra Gowda, C. D. and Nataraju, A. and Dhananjaya, B. L. and Kemparaju, K. and Vishwanath, B. S. (2005) Procoagulant activity of Calotropis gigantea latex associated with fibrin(ogen)olytic activity. Toxicon, 46 (1). 84 - 92. ISSN 1879-3150

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The latex of Calotropis gigantea is a rich source of useful components that has medicinal properties and one of the main applications is in controlling bleeding. The crude latex extract contained many proteins, which are highly basic in nature and exhibited strong proteolytic activity. The crude extract hydrolyses casein, human fibrinogen and crude fibrin clot in a dose-dependent manner. The hydrolyzing activity was completely inhibited by IAA indicating they belong to the super family, cysteine proteases. Crude extract hydrolyses Aα, Bβ and γ subunits of fibrinogen. Among all the subunits the preferential subunit to get hydrolyzed was Aα followed by Bβ and γ subunit is highly resistant and hydrolyzed at higher protein concentration or over a prolonged incubation time. The crude extract hydrolysis crude fibrin clot strongly compared to trypsin and papain. Pharmacologically the crude extract is hemorrhagic and induces skin hemorrhage at >75μg and reduces the coagulation time of citrated plasma from 150 to 47s and promotes blood coagulation. Procoagulation and blood clot hydrolysis are important in wound healing process. This is due to unique cysteine proteases of plant latex and is responsible for the pharmacological actions observed in folk medicine.

Item Type: Article
Uncontrolled Keywords: , Latex, Procoagulant activity, Fibrinogenolytic activity, Fibrinolytic activity, Hemostasis
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Manjula P Library Assistant
Date Deposited: 05 Sep 2019 10:03
Last Modified: 05 Sep 2019 10:03

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