PCR-based detection of phomopsis azadirachtae in die-back affected neem seeds

Girish, K. and Bhat, Shankara S. and Raveesha, K. A. (2009) PCR-based detection of phomopsis azadirachtae in die-back affected neem seeds. Archives of Phytopathology and Plant Protection, 42 (7). pp. 626-632. ISSN 1477-2906

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Official URL: http://doi.org/10.1080/03235400701287628

Abstract

Phomopsis azadirachtae Sateesh, Bhat & Devaki is the incitant of die-back disease of neem trees. Delayed appearance of conidia and presence of other microorganisms in the neem tissues are the obstacles in the rapid and accurate identification of P. azadirachtae. This work was carried out to develop a methodology for rapid detection of the pathogen in diseased tissues especially in the neem seeds. rDNA sequences of many Phomopsis spp. were retrieved from the database and were subjected for multiple alignment to select a 179 bp conserved sequence. This was used to design Phomopsis specific primer pair (Forward and Reverse) having the potential to produce a 154 bp product in PCR. The primer pair was utilised to detect the presence of P. azadirachtae in diseased neem seeds and other tissues. This is the first report on the PCR-based detection of P. azadirachtae directly in die-back diseased neem tissues. This method can be employed for rapid and reliable detection of P. azadirachtae in die-back affected neem seeds. Hence it will have very good application in seed health testing laboratories.

Item Type: Article
Uncontrolled Keywords: Die-back and neem and rDNA and P azadirachtae and PCR detection
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Shrirekha N
Date Deposited: 05 Sep 2019 05:58
Last Modified: 05 Sep 2019 05:58
URI: http://eprints.uni-mysore.ac.in/id/eprint/7597

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