In vitro evaluation of antagonistic microorganisms for the control of die-back of Neem causal agent phomopsis azadirachtae

Girish, K. and Bhat, S. S. and Raveesha, K. A. (2009) In vitro evaluation of antagonistic microorganisms for the control of die-back of Neem causal agent phomopsis azadirachtae. Journal of Plant Protection Research, 49 (4). ISSN 1899-007X

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Abstract

The die-back of neem caused by Phomopsis azadirachtae is a devastating disease in India reducing the life span and seed production of neem. Six isolates of antagonistic bacteria and fungi, Bacillus cereus (MTCC 430), Bacillus subtilis (MTCC 619), Pseudomonas aeruginosa (MTCC 2581), Pseudomonas oleovorans (MTCC 617), Trichoderma harzianum (MTCC 792) and Trichoderma viride (MTCC 800) were evaluated against P. azadirachtae under in vitro conditions. Culture filtrates of all these microorganisms were extracted using ethyl acetate, and the obtained fractions were tested for their antifungal activity against P. azadirachtae at different concentrations. Ethyl acetate extracts of B. subtilis and P. aeruginosa were highly effective and completely inhibited the growth of P. azadirachtae at 25 ppm concentration. Both these isolates may be considered as factors for the biological control of die-back of neem.

Item Type: Article
Uncontrolled Keywords: Bacillus subtilis and biological control and die-back of neem and Phomopsis azadirachtae and Pseudomonas aeruginosa
Subjects: B Life Science > Botany
Divisions: Department of > Botany
Depositing User: Shrirekha N
Date Deposited: 03 Sep 2019 06:33
Last Modified: 03 Sep 2019 06:33
URI: http://eprints.uni-mysore.ac.in/id/eprint/7479

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