Basavaraju, P. and Shailasree, S. and Nandini Shetty, P. and Kini, Ramachandra K. and Jorgensen, Hans J. L. and De Neergaard, Eigil and Shetty, H. S. (2009) Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction of colletotrichum sublineolum in sorghum. Journal of Plant Interactions, 4 (3). pp. 179-186. ISSN 1742-9145
Full text not available from this repository. (Request a copy)Abstract
Hydroxyproline-rich glycoproteins (HRGPs) accumulation and oxidative cross-linking is one of the earliest defense responses in plants against pathogen infection. In the present study HRGP accumulation in three sorghum genotypes i.e. SC146 (resistant), SC326 (intermediately resistant) and BTx623 (susceptible) as a response to Colletotrichum sublineolum isolate CP2126 infection is elucidated. HRGPs were monitored by hydroxyproline (Hyp) estimation. In genotypes SC146 and SC326 there was a significantly higher amounts of Hyp at 2 days after inoculation (dai) compared to genotype BTx623, indicating an infection induced accumulation of HRGPs. Western blot analysis of acid-ethanol extracted proteins with polyclonal antibody raised against pearl millet purified HRGPs identified four bands with molecular masses of similar to 65, 45, 17 and 14 kDa as HRGPs. Insolubilization of the 45 kDa protein in genotypes SC146 and SC326 upon infection with C. sublineolum indicates a role of this protein in cell wall cross-linking, coinciding with heavier accumulation of hydrogen peroxide. In addition, tissue print analysis using polyclonal antibody of pearl millet HRGPs recognized these cross-linked proteins to be HRGPs. These findings indicated that HRGPs in sorghum is a component of defense reaction against C. sublineolum infection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | sorghum anthracnose; disease resistance; hydroxyproline-rich glycoproteins; hydroxyproline; hydrogen peroxide |
| Subjects: | B Life Science > Biotechnology |
| Divisions: | Department of > Biotechnology |
| Depositing User: | Users 19 not found. |
| Date Deposited: | 30 Aug 2019 05:24 |
| Last Modified: | 30 Oct 2019 07:02 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/7350 |
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