Effect of anticoagulants on the plasma hyaluronidase activities

Sharma, R. and Mahadeswaraswamy, Y. H. and Harish Kumar, K. and Devaraja, S. and Kemparaju, K. and Vishwanath, B. S. and Girish, K. S. (2009) Effect of anticoagulants on the plasma hyaluronidase activities. Journal of Clinical Laboratory Analysis, 23 (1). pp. 29-33. ISSN 1098-2825

Full text not available from this repository. (Request a copy)
Official URL: http://doi.org/10.1002/jcla.20285


Mammalian hyaluronidases (HAases) are an endo-beta-N-acetyl-hexosaminidases that degrade hyaluronan (HA) and have been implicated in diverse pathophysiological functions. Several pathological conditions, such as diabetes, monoclonal gammapathy, and bladder and prostate tumors, report the distorted plasma HAase activity. However, the plasma HAase (hHyal-1) activity has been presumed to change with the circulating HA level and serves as an early marker for several diseases. It has been generally practised to use the anticoagulants such as tri-sodium citrate/disodium EDTA/heparin for the preparation of plasma for both biochemical and clinical analyses. In the present investigation, the effect of anticoagulants on plasma HAase activity was evaluated and compared with the serum HAase activity that is devoid of anticoagulants as no study provides information in this regard. The results suggested that the plasma HAase activity in the presence of the recommended concentration of EDTA was highly comparable/similar to that of the serum HAase activity. In contrast, citrated or heparinized plasma recorded a significantly reduced level of activity than that of the serum HAase activity. In conclusion, our results suggested that the EDTA-treated plasma samples are a better choice compared with heparin and citrated samples to assess the HAase activity. J. Clin. Lab. Anal. 23:29-33,2009. (C) 2009 Wiley-Liss, Inc.

Item Type: Article
Uncontrolled Keywords: Hyal-1; hyaluronidase; hyaluronan; anticoagulants; plasma; serum
Subjects: B Life Science > Biotechnology
C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Department of > Biotechnology
Depositing User: Users 19 not found.
Date Deposited: 29 Aug 2019 10:34
Last Modified: 29 Aug 2019 10:34
URI: http://eprints.uni-mysore.ac.in/id/eprint/7339

Actions (login required)

View Item View Item