A novel-approach to the establishment of dual cultures of pearl-millet and sclerospora-graminicola

Upadhyaya, G. and Shivanna, M. B. and Prakash, H. S. and Shetty, H. Shekar (1992) A novel-approach to the establishment of dual cultures of pearl-millet and sclerospora-graminicola. PLANT CELL TISSUE AND ORGAN CULTURE, 31 (3). pp. 203-206. ISSN 0167-6857

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Official URL: http://dx.doi.org/10.1007/BF00036224


Dual cultures were successfully established using malformed florets of pearl millet infected with Sclerospora graminicola, the downy mildew pathogen. A higher proportion (86%) of calli from malformed florets formed dual cultures on Murashige and Skoog's (MS) medium with 2 mg l-1 of 2,4-dichlorophenoxy acetic acid (2,4-D), Compared to shoot tips (25%). Fungal mycelium covered the entire surface of the callus within 30 days of placement of explants on the MS medium with 2 mg l-1 of 2,4-D. The infected calli also differentiated and produced plantlets when transferred to MS medium without 2,4-D.

Item Type: Article
Additional Information: Unmapped bibliographic data: DA - 1992/12// [EPrints field already has value set]
Subjects: Agriculture Biological Sciences > Biotechnology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biotechnology
Divisions: PG Campuses > Manasagangotri, Mysore > Biotechnology
Depositing User: Users 26 not found.
Date Deposited: 06 May 2013 10:40
Last Modified: 13 Aug 2013 06:19
URI: http://eprints.uni-mysore.ac.in/id/eprint/7322

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