Detection of fumonisin producing fusarium verticillioides in paddy (oryza sativa l.) using polymerase chain reaction (pcr)

Maheshwar, P. K. and Ahmed Moharram, S. and Janardhana, G. R. (2009) Detection of fumonisin producing fusarium verticillioides in paddy (oryza sativa l.) using polymerase chain reaction (pcr). Brazilian Journal of Microbiology, 40 (1). pp. 134-138. ISSN 1678-4405

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Official URL: http://doi.org/10.1590/S1517-83822009000100023

Abstract

The study reports the occurrence of fumonisin producing Fusarium verticillioides in 90 samples of stored paddy (Oryza sativa L.) collected from different geographical regions of Karnataka, India. Fumonisin producing F. verticillioides was identified based on micromorphological characteristics and PCR using two sets of primers. One set of primers was F. verticillioides species specific, which selectively amplified the intergenic space region of rDNA. The other set of primers was specific to fumonisin producing F. verticillioides. Eight paddy samples were positive for F. verticillioides. Eleven isolates obtained from these samples were capable of producing fumonisin.

Item Type: Article
Uncontrolled Keywords: Fusarium verticillioides; fumonisin; paddy; PCR
Subjects: B Life Science > Botany
B Life Science > Microbiology
Divisions: Department of > Botany
Yuvaraj college > Mircobiology
Depositing User: Shrirekha N
Date Deposited: 29 Aug 2019 06:16
Last Modified: 29 Aug 2019 06:16
URI: http://eprints.uni-mysore.ac.in/id/eprint/7300

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