Sudisha, J. and Ananda Kumar, S. and Niranjana, S. R. and Shetty, N. P. and Shetty, Shekar H. and Shekar H. (2009) Cloning and development of pathotype-specific SCAR marker associated with sclerospora graminicola isolates from pearl millet. Australasian Plant Pathology, 38 (3). pp. 216-221. ISSN 1448-6032
Full text not available from this repository. (Request a copy)Abstract
Downy mildew pathogen of pearl millet in India is associated with the spread of the highly virulent Sclerospora graminicola pathotype-1. Twenty-seven S. graminicola isolates were screened using 20 inter simple sequence repeats (ISSR). Dinucleotide repeat primer 17898A-(CA)(6) AC] amplified a similar to 600 bp fragment specific to five isolates of pathotype-1 (Sg 048, Sg 153, Sg 212, DM-11 and DM-90). The ISSR fragment linked with pathotype-1 was cloned successfully and sequenced. To convert ISSR fragments into pathotype-specific sequence characterised amplified region (SCAR) markers, PCR primers were designed using a sequence of the cloned DNA fragment. PCR amplification using SCAR primer pair (UOM3-Sg-Path1-F/R) amplified a single 284 bp band only in isolates of S. graminicola pathotype-1. This SCAR primer pair did not amplify the 284 bp product from the other five S. graminicola pathotypes or a negative control, which demonstrates primer specificity for pathotype-1. The SCAR primer pair (UOM3-Sg-Path1-F/R) obtained in this study will provide a valuable tool for rapid identification and specific detection of S. graminicola pathotype-1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PCR; Pennisetum glaucum; SCAR markers |
| Subjects: | B Life Science > Biotechnology |
| Divisions: | Department of > Biotechnology |
| Depositing User: | Users 19 not found. |
| Date Deposited: | 29 Aug 2019 06:01 |
| Last Modified: | 25 Oct 2019 09:54 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/7296 |
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