Adkar-Purushothama, C. R. and Quaglino, Fabio and Paola Casati and Janardhana, G. R. and Bianco, P. A. (2009) Genetic diversity among `candidatus liberibacter asiaticus' isolates based on single nucleotide polymorphisms in 16s rrna and ribosomal protein genes. Annals of Microbiology, 59 (4). pp. 681-688. ISSN 1869-2044
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Citrus Huanglongbing (HLB) is one of the most destructive and widespread diseases of citrus, caused by `Candidatus Liberibacter', a non-cultured, phloem-restricted alpha-proteobacterium. In the present study, `Ca. Liberibacter' was detected in symptomatic citrus plants growing in the Karnataka state (South India) by amplification of 16S rRNA and P-operon ribosomal protein (beta-rp) genes, using PCR primers specific for African and Asian `Ca. Liberibacter'. Sequencing of the 16S rRNA amplified fragment revealed that `Ca. Liberibacter', here identified belongs to the species `Ca. Liberibacter asiaticus'. The alignments of 16S rRNA and ribosomal protein gene sequences of all known `Ca. Liberibacter asiaticus'-related, allowed to recognize Single Nucleotide Polymorphisms (SNPs). Basing on SNP analysis, the strains were grouped in fourteen 16SrRNA SNP genetic lineages (16Sr-I to 16Sr-XIV) and three beta-rp SNP genetic lineages (rp-I to rp-III). Only the strains of `Ca. Liberibacter asiaticus' from the Karnataka state belonged to the genetic lineages 16Sr-I and rp-I. RFLP diagnostic tests on the discriminative 16S rRNA SNPs were set up to identify this lineage. These results revealed the presence of a new `Ca. Liberibacter asiaticus' genetic lineage in the Indian sub-continent, where at least four genetically diverse SNP lineages were found. These findings could open new opportunities for in-depth studies on biological niches and traits of `Ca. Liberibacter asiaticus'.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Huanglongbing (HLB); `Candidatus Liberibacter'; single nucleotide polymorphisms; genetic lineage |
| Subjects: | B Life Science > Botany |
| Divisions: | Department of > Botany |
| Depositing User: | Users 19 not found. |
| Date Deposited: | 28 Aug 2019 10:33 |
| Last Modified: | 18 Jul 2022 05:46 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/7275 |
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