Ultraviolet b radiation generated platelet-activating factor receptor agonist formation involves egf-r-mediated reactive oxygen species

Yao, Yongxue and Wolverton, Jay E. and Zhang, Qiwei and Marathe, Gopal K. and Al-Hassani, Mohammed and Konger, Raymond L. and Travers, Jeffrey B. (2009) Ultraviolet b radiation generated platelet-activating factor receptor agonist formation involves egf-r-mediated reactive oxygen species. Journal of Immunology, 182 (5). pp. 2842-2848. ISSN 1550-6606

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Official URL: http://doi.org/10.4049/jimmunol.0802689

Abstract

Recent studies have implicated the lipid mediator platelet-activating factor (PAF) in UVB-mediated systemic immunosuppression known to be a major cause for skin cancers. Previously, our group has demonstrated that UVB irradiation triggers the production of PAF and oxidized glycerophosphocholines that act as PAF-receptor (PAF-R) agonists. The present studies explored the mechanisms by which UVB generates PAF-R agonists. UVB irradiation of human epidermal KB cells resulted in both increased levels of reactive oxygen species (ROS) and PAF-R agonistic activity. Pretreatment of KB cells with antioxidants vitamin C and N-acetylcysteine or the pharmacological inhibitor PD168393 specific for the epidermal growth factor receptor all inhibited UVB-induced ROS as well as PAF-R agonists, yet had no effect on fMLP-mediated PAF-R agonist production. In addition, in vivo production of PAF-R agonists from UVB-irradiated mouse skin was blocked by both systemic vitamin C administration and topical PD168393 application. Moreover, both vitamin C and PD168393 abolished UVB-mediated but not the PAF-R agonist 1-hexadecyl-2-N-methylcarbamoyl glycerophosphocholine-mediated immunosuppression as measured by the inhibition of delayed type contact hypersensitivity to the chemical dinitrofluorobenzene. These studies suggest that UVB-induced systemic immunosuppression is due to epidermal growth factor receptor-mediated ROS which results in PAF-R agonist formation. The Journal of Immunology, 2009, 182: 2842-2848.

Item Type: Article
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Shrirekha N
Date Deposited: 28 Aug 2019 06:19
Last Modified: 28 Aug 2019 06:19
URI: http://eprints.uni-mysore.ac.in/id/eprint/7216

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