Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-based DNA barcoding tools

Bharathi Ramasetty, T. and Bajpe, Shrisha Naik and Sampath Kumara, K. K. and Saini, Ramesh Kumar and Shashibhushan, N. B. and Ramachandra, K. K. and Prakash, H. S. (2016) Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-based DNA barcoding tools. Electronic Journal of Biotechnology, 24. 1 - 8.

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Official URL: https://doi.org/10.1016/j.ejbt.2016.09.001

Abstract

Background Memecylon species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psbA-trnH, rpoC1, ndh and atpF-atpH, were used to authenticate and analyze the diversity of five Memecylon species collected from Western Ghats of India. Results Phylogenetic analysis clearly distinguished Memecylon malabaricum from Memecylon wightii and Memecylon umbellatum from Memecylon edule and clades formed are in accordance with morphological keys. In the RAPD and ISSR analyses, 27 accessions representing five Memecylon species were distinctly separated into three different clades. M. malabaricum and M. wightii grouped together and M. umbellatum, M. edule and Memecylon talbotianum grouped in the same clade with high Jaccard dissimilarity coefficient and bootstrap support between each node, indicating that these grouped species are phylogenetically similar. Conclusion Data from the present study reveals that chloroplast psbA-trnH region could be used as a potential candidate region for identifying Memecylon species, and ISSR marker system could be used for estimating genetic diversity since it has high percent polymorphism compared to RAPD marker.

Item Type: Article
Uncontrolled Keywords: FH, species, PCR based techniques, Molecular phylogenetic analysis, Molecular phylogenesis, DNA markers
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: manjula User
Date Deposited: 30 May 2019 11:34
Last Modified: 05 Jul 2019 09:43
URI: http://eprints.uni-mysore.ac.in/id/eprint/709

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