A pcr-based assay for the detection and differentiation of potential fumonisin-producing fusarium verticillioides isolated from Indian maize kernels

Sreenivasa, M. Y. and María Teresa González, Jaen and Sharmila Dass, R. and Charith Raj, A. P. and Janardhana, G. R. (2008) A pcr-based assay for the detection and differentiation of potential fumonisin-producing fusarium verticillioides isolated from Indian maize kernels. Food Biotechnology, 22 (2). pp. 160-170. ISSN 1532-4249

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Official URL: https://doi.org/10.1080/08905430802043206

Abstract

One hundred and three Fusarium isolates from maize samples collected from different districts of Karnataka state, India, were analyzed with genus-specific, species-specific, and potential fumonisin specific oligonucleotide primers. One set of genus-specific primers ITS F and ITS R based on a highly conserved ITS region of the genus Fusarium were used to differentiate Fusarium species from closely related genera. All the Fusarium species tested scored positive with the ITS pair of primers. Detection and identification of Fusarium verticillioides species was done by using a newly designed reverse primer VERT-R (5′- CGA CTC ACG GCC AGG AAA CC −3′) based on an intergenic spacer sequence (IGS) combined with an already designed forward primer VERTF-1 (5′-GCG GGA ATT CAA AAG TGG CC -3′) published previously. Out of 103 Fusarium species tested, 83 isolates of F. verticillioides scored positive for VERTF-1/ VERT-R species-specific pair of primers. Further to discriminate potential fumonisin-producing and nonproducing strains of F. verticillioides, the VERTF-1/VERTF-2 set of primers [VERTF-1 (5′-GCG GGA ATT CAA AAG TGG CC -3′) and VERTF-2 (5′-GAG GGC GCG AAA CGG ATC GG -3′)] were used. 64 isolates of F. verticillioides scored positive for VERTF-1/ VERTF-2 pair of primers. In total, three primers, one forward primer VERTF-1 and two reverse primers VERT-R and VERTF-2, were used for the confirmation of F. verticillioides up to the species level and the second pair of primers were used to confirm the potential for fumonisin production. The developed PCR assay should provide a powerful tool for the detection and differentiation of potential fumonisin-producing F. verticillioides strains in a population.

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: manjula User
Date Deposited: 26 Aug 2019 11:30
Last Modified: 26 Aug 2019 11:30
URI: http://eprints.uni-mysore.ac.in/id/eprint/7080

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