Radfar, M. and Sudarshana, M. S. and Niranjan, M. H. (2012) Betalains from stem callus cultures of Zaleya Decandra L. N. Burm. F. -a medicinal herb. Journal of Medicinal Plant Research, 6 (2). pp. 2443-2447. ISSN 1996-0875
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The plant synthesizes various medicinal important compounds; similarly in vitro derived callus also synthesizes the compounds. Hence, callus can also be used as an alternative to whole plant for the production of secondary metabolites. A preliminary experiment was conducted using stem explants of Zaleya decandra (Aizoaceae) on MS, B5 and Whites media containing different concentrations of auxins and cytokinins in order to test the best suitable medium for callogenic potentiality. Murashige and Skoog (MS) medium elicited a better response than other media used. The callus formation and the nature of callus varies depending upon the nature of the growth regulators used. The various concentrations and combinations of dichlorophenoxy acetic acid (2,4-D), 6-benzylaminopurine (BAP), thidiazuron(TDZ), kinetin (Kn), naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and coconut milk were tested. Of these, combination of 2,4-D (1.0 mg/L) and TDZ (2.0 mg/L) was found to be the best in inducing pigmented callus. As the concentrations of 2,4-D and TDZ increased upto 5 mgL-1, callogenic potentiality of the explants decreased. Phenotype color ranged from white/green through yellow, mezenda, violet red to red representing different types of pigments. The pigment when subjected to laboratory test using a spectrophotometer confirmed as betalains.
| Item Type: | Article |
|---|---|
| Subjects: | B Life Science > Botany |
| Divisions: | Department of > Botany |
| Depositing User: | C Swapna Library Assistant |
| Date Deposited: | 26 Aug 2019 09:47 |
| Last Modified: | 26 Aug 2019 09:47 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/7069 |
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