Madhu Kumar, A. V. and Veerabasappa Gowda, T. (2006) Novel non-enzymatic toxic peptide of Daboia russelii (Eastern region) venom renders commercial polyvalent antivenom ineffective. Toxicon, 47 (4). 398 - 408. ISSN 1879-3150
Full text not available from this repository. (Request a copy)Abstract
The snake venoms are typically complex mixtures of enzymes and non-enzymatic peptides. Regional variation in the non-enzymatic fraction of Russell's viper venom from three regions of India studied. The eastern, western and southern regional venom upon gel permeation chromatography on sephadex-G-75 column resolved into three peaks. All the three overlapping peaks differ in their lethality and enzymatic potency. Peak III of all the regional venom found to be non-enzymatic, Western and southern regional venom has trypsin inhibitory activity with varying potencies. Interestingly, the peak III of eastern region is devoid of trypsin inhibitory activity. But it is highly lethal with a LD50 0.7mg/kg body weight and also it exhibited post-synaptic neurotoxicity. On the other hand southern and western regional venom's non-enzymatic peak is non-lethal and did not induce neurotoxic symptoms in experimental model. The antibodies developed against the eastern regional venom cross-reacted with the peaks I and II of other regional venom, but failed to cross-react with the peak III of western and southern regional Russell's viper venom. Commercial anti-venom prepared to neutralize the toxic effects of common poisonous snakes of India, showed positive cross-reaction against peaks I, II and III of all three regional venom tested, except peak III of eastern regional venom. Commercial anti-venom neutralized the lethal toxicity of both western and southern regional Russell's viper venom, and failed to neutralize the lethal effects of eastern regional Russell's viper venom.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Non-enzymatic peptides, Trypsin inhibitors, Neurotoxicity, Regional variations, venom, Immunological cross-reaction |
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | LA manjunath user |
| Date Deposited: | 26 Aug 2019 06:18 |
| Last Modified: | 23 Dec 2019 10:12 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/7021 |
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