Nagaraja, P. and Shivakumar, A. and Shrestha, Ashwinee Kumar (2009) Peroxidase-catalyzed oxidative coupling of paraphenylenediamine with 3-dimethylaminobenzoic acid: application in crude plant extracts. Journal of Agricultural and Food Chemistry, 57 (12). pp. 5173-5177. ISSN 1520-5118
Full text not available from this repository. (Request a copy)Abstract
This paper presents a novel spectrophotometric method to measure peroxidase activity using paraphenylenediamine dihydrochloride (PPDD) and 3-dimethylaminobenzoic acid (DMAB). The PPDD traps free radicals and becomes oxidized to electrophillic 1,4-diimine, which couples with DMAB to give an intense green-colored chromogenic species with maximum absorbance at 710 nm. This assay was adopted for the quantification of hydrogen peroxide between 5 and 45 mu M. From the kinetic data, a two-substrate ping-pong mechanism of peroxidase was established. The catalytic efficiency and catalytic constant (k(cat)) of the proposed assay were 0.54 x 10(6) M-1 min(-1) and 0.0436 x 10(3) min(-1), respectively. As a simple, rapid, precise, and sensitive technique, PPDD-DMAB stands as a potential replacement for the traditional guaiacol method. Application of this method in plant extracts opens its relevance in the field of biochemical analysis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Horseradish peroxidase; PPDD; DMAB; catalytic parameters; crude extracts |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Users 19 not found. |
| Date Deposited: | 23 Aug 2019 09:35 |
| Last Modified: | 23 Aug 2019 09:35 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/6963 |
Actions (login required)
 |
View Item |
