Induction of antioxidant enzymes associated with bacterial spot pathogenesis in tomato

Chandrashekar, S. and Umesha, S. (2012) Induction of antioxidant enzymes associated with bacterial spot pathogenesis in tomato. International Journal of Food, Agriculture and Veterinary Sciences, 2 (2). pp. 22-34. ISSN 2277-209X

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Abstract

Plant–pathogen interactions are mediated by a complex network of molecular and cytological events that determine a range between susceptibility and resistance. The production of reactive oxygen species, via consumption of oxygen so-called oxidative burst, is one of the earliest cellular responses following successful pathogen recognition. The present investigation was focused on the role of antioxidant enzymes in imparting resistance in tomato against bacterial spot pathogenXanthomonas axonopodis pv.vesicatoria. Ten different tomato cultivars were collected from private seed agencies and screened for resistance to bacterial spot disease, using artificial inoculation technique under greenhouse conditions. Involvement of antioxidant enzymes in bacterial spot pathogenesis was studied in resistant, susceptible and highly susceptible tomato cultivars. Eight-day-old seedlings were root-dip and spray inoculated with the inoculum (1 x 108cfu/ml) and harvested at different time intervals (0, 3, 6, 9, 12, 15, etc., up to 72 h) and assayed for two antioxidant enzymes. Temporal accumulation of ascorbate peroxidase and catalase enzymes showed maximum activity at 12 and 21h after pathogen inoculation (hpi) in resistant cultivar, whereas in susceptible and highly susceptible cultivars it increased at 18 and 36h for ascorbate peroxidase and 42 and 54 h for catalase respectively. Furthermore, Isoforms analysis of APX and CAT enzymes indicated the clear difference between resistant and susceptible cultivars. Resistant cultivar showed higher enzyme activity after pathogen inoculation when compared to uninoculated control and also the susceptible cultivars. The role of these two antioxidant enzymes in imparting resistance to tomato against bacterial spot pathogenesis is discussed.

Item Type: Article
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: MUL SWAPNA user
Date Deposited: 23 Aug 2019 06:05
Last Modified: 23 Aug 2019 06:05
URI: http://eprints.uni-mysore.ac.in/id/eprint/6948

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