Evaluation of antioxidant and antibacterial activities of methanolic flower extract of Wedelia trilobata (L.) Hitch

Chethan, J. and Sampath Kumara, K. K. and Niranjana, S. R. and Prakash, H. S. (2012) Evaluation of antioxidant and antibacterial activities of methanolic flower extract of Wedelia trilobata (L.) Hitch. African Journal of Biotechnology, 11 (41). pp. 9829-9834. ISSN 1684-5315

[img] Text (Full Text)
Evaluation of Antioxidant and Antibacterial Activities.pdf - Published Version

Download (169kB)
Official URL: https://doi.org/10.5897/AJB11.3729

Abstract

Wedelia trilobata (L.) Hitch is a member of the family Asteraceae, with attractive yellow flowers borne singly at the end of each stem. The work was conducted to investigate the antioxidant, antimicrobial and DNA protecting ability of the methanol extract of W. trilobata flower. Antioxidant properties were assessed by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis 3- thylbenzthiazoline-6-sulphonic acid (ABTS) assay. The methanol extract and standard ascorbic acid showed antioxidant activity with IC50 value of 90 μg/ml and 60 μg/ml respectively in DPPH radical scavenging assay. The in vitro antibacterial screening was evaluated by disc diffusion method against three Gram-positive and three Gram-negative human pathogenic bacteria. The extract showed zones of inhibition of 10-16 mm against different strains.The phenolic content of the extract was 250 gallic acid equivalence (GAE in µg) whichwas measured by Folin-Ciocalteu method. The extract was tested for pTZ57R/T plasmid DNA protection against hydroxyl radicals as evidenced by DNA fragmentation assay.

Item Type: Article
Uncontrolled Keywords: Asteraceae plant species, methanol extract, antioxidant, antimicrobial, DNA protection assay
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: MUL SWAPNA user
Date Deposited: 23 Aug 2019 05:07
Last Modified: 23 Aug 2019 05:07
URI: http://eprints.uni-mysore.ac.in/id/eprint/6923

Actions (login required)

View Item View Item