Nitric oxide is involved in chitosan-induced systemic resistance in pearl millet against downy mildew disease

Manjunatha, G. and Niranjan Raj, S. and Prashanth, G. N. and Deepak, S. and Amruthesh, K. N. and Shetty, H. S. (2009) Nitric oxide is involved in chitosan-induced systemic resistance in pearl millet against downy mildew disease. Pest Management Science, 65 (7). pp. 737-743. ISSN 1526-4998

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BACKGROUND: The nature and durability of resistance offered by chitosan and the involvement of nitric oxide (NO) in chitosan-induced defence reactions in pearl millet against downy mildew disease were investigated. RESULTS: It had previously been reported that chitosan seed priming protected pearl millet plants against downy mildew disease. Further elucidation of the mechanism of resistance showed that chitosan seed priming protects the plants systemically. A minimum 4 day time gap is required between the chitosan treatment and pathogen inoculation for maximum resistance development, and it was found to be durable. Chitosan seed priming elevated NO accumulation in pearl millet seedlings, beginning from 2 h post-inoculation, and it was found to be involved in the activation of early defence reactions such as hypersensitive reaction, callose deposition and PR-1 protein expression. Pretreatment with NO scavenger C-PTIO and nitric oxide synthase (NOS) inhibitor L-NAME before pathogen inoculation reduced the disease-protecting ability of chitosan, and defence reactions were also downregulated, which indicated a possible role for NO in chitosan-induced resistance. CONCLUSION: Protection offered by chitosan against pearl millet downy mildew disease is systemic in nature and durable. Chitosan-induced resistance is activated via NO signalling, as defence reactions induced by chitosan were downregulated under NO deficient conditions. (C) 2009 Society of Chemical Industry

Item Type: Article
Uncontrolled Keywords: nitric oxide; chitosan; pearl millet; Sclerospora graminicola; downy mildew
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Users 19 not found.
Date Deposited: 22 Aug 2019 10:13
Last Modified: 22 Dec 2020 05:34

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